Ontology highlight
ABSTRACT:
SUBMITTER: Harris LC
PROVIDER: S-EPMC329112 | biostudies-other | 1991 Nov
REPOSITORIES: biostudies-other
Harris L C LC Potter P M PM Tano K K Shiota S S Mitra S S Brent T P TP
Nucleic acids research 19911101 22
O6-methylguanine-DNA methyltransferase (MGMT) is a ubiquitous protein responsible for repair of O6-alkylguanine, a mutagenic, carcinogenic and toxic lesion. To characterize the elements responsible for the regulation of the MGMT gene, a 2.6 kb Sstl fragment isolated from a genomic clone, was shown to contain 5' flanking sequences of the gene. The promoter activity of this fragment as well as various subfragments were tested in NIH 3T3 mouse fibroblasts by transient expression of the bacterial ch ...[more]