Ontology highlight
ABSTRACT:
SUBMITTER: Brumbaugh J
PROVIDER: S-EPMC3358887 | biostudies-other | 2012 May
REPOSITORIES: biostudies-other
Brumbaugh Justin J Hou Zhonggang Z Russell Jason D JD Howden Sara E SE Yu Pengzhi P Ledvina Aaron R AR Coon Joshua J JJ Thomson James A JA
Proceedings of the National Academy of Sciences of the United States of America 20120402 19
The transcription factor OCT4 is fundamental to maintaining pluripotency and self-renewal. To better understand protein-level regulation of OCT4, we applied liquid chromatography-MS to identify 14 localized sites of phosphorylation, 11 of which were previously unknown. Functional analysis of two sites, T234 and S235, suggested that phosphorylation within the homeobox region of OCT4 negatively regulates its activity by interrupting sequence-specific DNA binding. Mutating T234 and S235 to mimic co ...[more]