Unknown

Dataset Information

0

Handling of artificial membranes using electrowetting-actuated droplets on a microfluidic device combined with integrated pA-measurements.


ABSTRACT: Artificial membranes, as a controllable environment, are an essential tool to study membrane proteins. Electrophysiology provides information about the ion transport mechanism across a membrane at the single-protein level. Unfortunately, high-throughput studies and screening are not accessible to electrophysiology because it is a set of not automated and technically delicate methods. Therefore, it is necessary to automate and parallelize electrophysiology measurement in artificial membranes. Here, we present a first step toward this goal: the fabrication and characterization of a microfluidic device integrating electrophysiology measurements and the handling of an artificial membrane which includes its formation, its displacement and the separation of its leaflets using electrowetting actuation of sub-μL droplets. To validate this device, we recorded the insertion of a model porin, α-hemolysin.

SUBMITTER: Martel A 

PROVIDER: S-EPMC3365332 | biostudies-other | 2012 Mar

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC5036579 | biostudies-literature
| S-EPMC6189997 | biostudies-literature
| S-EPMC5073376 | biostudies-literature
| S-EPMC6190408 | biostudies-literature
| S-EPMC5511244 | biostudies-literature
| S-EPMC5770462 | biostudies-literature
| S-EPMC6187503 | biostudies-literature
| S-EPMC6405956 | biostudies-literature
| S-EPMC3357545 | biostudies-literature
| S-EPMC3570777 | biostudies-literature