Unknown

Dataset Information

0

Rapid fabrication of a microdevice with concave microwells and its application in embryoid body formation.


ABSTRACT: Here, we report a novel method for the fabrication of polydimethylsiloxane microdevices with complicated 3-D structures, such as concave and crater shapes, using an easily machined polymethyl methacrylate mold combined with a one-step molding process. The procedure presented here enables rapid preparation of complex 3-D microstructures varying in shape and dimensions. To regulate embryoid body (EB) formation, we fabricated a microfluidic device with an array of concave microwells and found that EBs growing in microwells maintained their shape, viability, and a high degree of homogeneity. We believe that this novel method provides an alternative for rapid prototyping, especially in fabricating devices with curved 3-D microstructures.

SUBMITTER: Xu Y 

PROVIDER: S-EPMC3365352 | biostudies-other | 2012 Mar

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC4978968 | biostudies-literature
| S-EPMC4439309 | biostudies-literature
| S-EPMC4426716 | biostudies-literature
| S-EPMC6102628 | biostudies-literature
| S-EPMC6272923 | biostudies-literature
| S-EPMC10855324 | biostudies-literature
| S-EPMC4217711 | biostudies-literature
| S-EPMC2755612 | biostudies-literature
| S-EPMC7855688 | biostudies-literature
| S-EPMC3787388 | biostudies-literature