Ontology highlight
ABSTRACT:
SUBMITTER: Owen SC
PROVIDER: S-EPMC3423826 | biostudies-other | 2012 Aug
REPOSITORIES: biostudies-other
Owen Shawn C SC Doak Allison K AK Wassam Pascal P Shoichet Molly S MS Shoichet Brian K BK
ACS chemical biology 20120608 8
Many small molecules, including bioactive molecules and approved drugs, spontaneously form colloidal aggregates in aqueous solution at micromolar concentrations. Though it is widely accepted that aggregation leads to artifacts in screens for ligands of soluble proteins, the effects of colloid formation in cell-based assays have not been studied. Here, seven anticancer drugs and one diagnostic reagent were found to form colloids in both biochemical buffer and in cell culture media. In cell-based ...[more]