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Mps1 promotes rapid centromere accumulation of Aurora B.

ABSTRACT: Aurora B localization to mitotic centromeres, which is required for proper chromosome alignment during mitosis, relies on Haspin-dependent histone H3 phosphorylation and on Bub1-dependent histone H2A phosphorylation--which interacts with Borealin through a Shugoshin (Sgo) intermediate. We demonstrate that Mps1 stimulates the latter recruitment axis. Mps1 activity enhances H2A-T120ph and is critical for Sgo1 recruitment to centromeres, thereby promoting Aurora B centromere recruitment in early mitosis. Importantly, chromosome biorientation defects caused by Mps1 inhibition are improved by restoring Aurora B centromere recruitment. As Mps1 kinetochore localization reciprocally depends on Aurora B, we propose that this Aurora B-Mps1 recruitment circuitry cooperates with the Aurora B-Haspin feedback loop to ensure rapid centromere accumulation of Aurora B at the onset of mitosis.

SUBMITTER: van der Waal MS

PROVIDER: S-EPMC3432816 | biostudies-other | 2012 Sep

REPOSITORIES: biostudies-other

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Mps1 promotes rapid centromere accumulation of Aurora B.

van der Waal Maike S MS Saurin Adrian T AT Vromans Martijn J M MJ Vleugel Mathijs M Wurzenberger Claudia C Gerlich Daniel W DW Medema René H RH Kops Geert J P L GJ Lens Susanne M A SM

EMBO reports 20120626 9

Aurora B localization to mitotic centromeres, which is required for proper chromosome alignment during mitosis, relies on Haspin-dependent histone H3 phosphorylation and on Bub1-dependent histone H2A phosphorylation--which interacts with Borealin through a Shugoshin (Sgo) intermediate. We demonstrate that Mps1 stimulates the latter recruitment axis. Mps1 activity enhances H2A-T120ph and is critical for Sgo1 recruitment to centromeres, thereby promoting Aurora B centromere recruitment in early mi ...[more]

PMID: 22732840

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