Ontology highlight
ABSTRACT:
SUBMITTER: Lee KC
PROVIDER: S-EPMC3507232 | biostudies-other | 2012 Sep
REPOSITORIES: biostudies-other
Lee Ka Cheong KC Padget Kay K Curtis Hannah H Cowell Ian G IG Moiani Davide D Sondka Zbyslaw Z Morris Nicholas J NJ Jackson Graham H GH Cockell Simon J SJ Tainer John A JA Austin Caroline A CA
Biology open 20120711 9
Topoisomerase II creates a double-strand break intermediate with topoisomerase covalently coupled to the DNA via a 5'-phosphotyrosyl bond. These intermediate complexes can become cytotoxic protein-DNA adducts and DSB repair at these lesions requires removal of topoisomerase II. To analyse removal of topoisomerase II from genomic DNA we adapted the trapped in agarose DNA immunostaining assay. Recombinant MRE11 from 2 sources removed topoisomerase IIα from genomic DNA in vitro, as did MRE11 immuno ...[more]