Ontology highlight
ABSTRACT:
SUBMITTER: Holland AJ
PROVIDER: S-EPMC3523849 | biostudies-other | 2012 Dec
REPOSITORIES: biostudies-other
Holland Andrew J AJ Fachinetti Daniele D Han Joo Seok JS Cleveland Don W DW
Proceedings of the National Academy of Sciences of the United States of America 20121112 49
Inducible degradation is a powerful approach for identifying the function of a specific protein or protein complex. Recently, a plant auxin-inducible degron (AID) system has been shown to degrade AID-tagged target proteins in nonplant cells. Here, we demonstrate that an AID-tagged protein can functionally replace an endogenous protein depleted by RNAi, leading to an inducible null phenotype rapidly after auxin addition. The AID system is shown to be capable of controlling the stability of AID-ta ...[more]