Ontology highlight
ABSTRACT:
SUBMITTER: Bierbaum M
PROVIDER: S-EPMC3627872 | biostudies-other | 2013 Apr
REPOSITORIES: biostudies-other
Bierbaum Martin M Bastiaens Philippe I H PI
Biophysical journal 20130401 8
The formation of an activity gradient of the small G-protein Ran around chromatin depends on the differential partitioning of the opposing enzyme activities of the Ran guanine nucleotide exchange factor RCC1 that resides on chromatin, and the cytoplasmic Ran GTPase activating protein RanGAP. We studied the time-dependent interaction kinetics between RCC1 and chromatin and the mobility of the Ran-RCC1 complex in living cells by fluorescence correlation spectroscopy to investigate whether binding ...[more]