Neutron-encoded protein quantification by peptide carbamylation.
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ABSTRACT:
SUBMITTER: Ulbrich A
PROVIDER: S-EPMC3880647 | biostudies-other | 2014 Jan
REPOSITORIES: biostudies-other
Publications
Neutron-encoded protein quantification by peptide carbamylation.
Ulbrich Arne A Merrill Anna E AE Hebert Alexander S AS Westphall Michael S MS Keller Mark P MP Attie Alan D AD Coon Joshua J JJ
Journal of the American Society for Mass Spectrometry 20131029 1
We describe a chemical tag for duplex proteome quantification using neutron encoding (NeuCode). The method utilizes the straightforward, efficient, and inexpensive carbamylation reaction. We demonstrate the utility of NeuCode carbamylation by accurately measuring quantitative ratios from tagged yeast lysates mixed in known ratios and by applying this method to quantify differential protein expression in mice fed a either control or high-fat diet. ...[more]
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