Ontology highlight
ABSTRACT:
SUBMITTER: Gregoire C
PROVIDER: S-EPMC38957 | biostudies-other | 1996 Jul
REPOSITORIES: biostudies-other
Grégoire C C Lin S Y SY Mazza G G Rebai N N Luescher I F IF Malissen B B
Proceedings of the National Academy of Sciences of the United States of America 19960701 14
We used stepwise photochemical cross-linking for specifically assembling soluble and covalent complexes made of a T-cell antigen receptor (TCR) and a class I molecule of the major histocompatibility complex (MHC) bound to an antigenic peptide. For that purpose, we have produced in myeloma cells a single-chain Fv construct of a TCR specific for a photoreactive H-2Kd-peptide complex. Photochemical cross-linking of this TCR single-chain Fv with a soluble form of the photoreactive H-2Kd-peptide liga ...[more]