Ontology highlight
ABSTRACT:
SUBMITTER: Radecke F
PROVIDER: S-EPMC394696 | biostudies-other | 1995 Dec
REPOSITORIES: biostudies-other
Radecke F F Spielhofer P P Schneider H H Kaelin K K Huber M M Dötsch C C Christiansen G G Billeter M A MA
The EMBO journal 19951201 23
A system has been established allowing the rescue of replicating measles viruses (MVs) from cloned DNA. On one hand, plasmids were constructed from which MV antigenomic RNAs with the correct termini are transcribed by phage T7 RNA polymerase. On the other hand, helper cells derived from the human embryonic kidney 293 cell line were generated constitutively expressing T7 RNA polymerase together with MV nucleocapsid protein and phosphoprotein. Simultaneous transfection of the helper cells with the ...[more]