Ontology highlight
ABSTRACT:
SUBMITTER: Sysoeva TA
PROVIDER: S-EPMC4040557 | biostudies-other | 2014 May
REPOSITORIES: biostudies-other
Proceedings of the National Academy of Sciences of the United States of America 20140514 21
Protein secretion typically involves translocation of unfolded polypeptides or transport of monomeric folded proteins. Here we provide, to our knowledge, the first experimental evidence for secretion of an intact multimeric complex requiring a signal formed by both members of the complex. Using systematic mutagenesis of a substrate involved in early secretory antigen 6 kDa (ESX) secretion in Bacillus subtilis, we demonstrate that export of the substrate requires two independent motifs. Using mix ...[more]