Dataset Information

A comparative analysis of genetic variation in rootstocks and scions of old olive trees - a window into the history of olive cultivation practices and past genetic variation.

ABSTRACT: BACKGROUND: Past clonal propagation of olive trees is intimately linked to grafting. However, evidence on grafting in ancient trees is scarce, and not much is known about the source of plant material used for rootstocks. Here, the Simple Sequence Repeat (SSR) marker technique was used to study genetic diversity of rootstocks and scions in ancient olive trees from the Levant and its implications for past cultivation of olives. Leaf samples were collected from tree canopies (scions) and shoots growing from the trunk base (suckers). A total of 310 trees were sampled in 32 groves and analyzed with 14 SSR markers. RESULTS: In 82.7% of the trees in which both scion and suckers could be genotyped, these were genetically different, and thus suckers were interpreted to represent the rootstock of grafted trees. Genetic diversity values were much higher among suckers than among scions, and 194 and 87 multi-locus genotypes (MLGs) were found in the two sample groups, respectively. Only five private alleles were found among scions, but 125 among suckers. A frequency analysis revealed a bimodal distribution of genetic distance among MLGs, indicating the presence of somatic mutations within clones. When assuming that MLGs differing by one mutation are identical, scion and sucker MLGs were grouped in 20 and 147 multi-locus lineages (MLLs). The majority of scions (90.0%) belonged to a single common MLL, whereas 50.5% of the suckers were single-sample MLLs. However, one MLL was specific to suckers and found in 63 (22.6%) of the samples. CONCLUSIONS: Our results provide strong evidence that the majority of olive trees in the study are grafted, that the large majority of scions belong to a single ancient cultivar containing somatic mutations, and that the widespread occurrence of one sucker genotype may imply rootstock selection. For the majority of grafted trees it seems likely that saplings were used as rootstocks; their genetic diversity probably is best explained as the result of a long history of sexual reproduction involving cultivated, feral and wild genotypes.

SUBMITTER: Barazani O

PROVIDER: S-EPMC4049413 | biostudies-other | 2014

REPOSITORIES: biostudies-other

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A comparative analysis of genetic variation in rootstocks and scions of old olive trees - a window into the history of olive cultivation practices and past genetic variation.

Barazani Oz O Westberg Erik E Hanin Nir N Dag Arnon A Kerem Zohar Z Tugendhaft Yizhar Y Hmidat Mohammed M Hijawi Thameen T Kadereit Joachim W JW

BMC plant biology 20140528

<h4>Background</h4>Past clonal propagation of olive trees is intimately linked to grafting. However, evidence on grafting in ancient trees is scarce, and not much is known about the source of plant material used for rootstocks. Here, the Simple Sequence Repeat (SSR) marker technique was used to study genetic diversity of rootstocks and scions in ancient olive trees from the Levant and its implications for past cultivation of olives. Leaf samples were collected from tree canopies (scions) and sho ...[more]

PMID: 24886387

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Project description:IntroductionThe Mediterranean basin is home to centuries-old large olive trees; high-vigor cultivars are widespread, with training forms poorly adapted to mechanical harvesting by trunk/branch shakers. The significant quantity of leaves, the considerable tree height, and the presence of multiple dichotomous hanging branches reduce the transmission of vibrations applied by the branch-shaker machines. Thus, re-shaping pruning may improve the performance of this modern mechanical harvesting method by focusing on removing both the hanging branches and those forming dichotomies. The goal of this study was to evaluate the dynamic responses of large-sized olive trees to pruning (or not) through various field tests under different excitation forces. We hypothesized that more rational pruning could significantly increase vibration transmissions.MethodsTo assess the transmission of vibrations, tests were conducted before and after the pruning on representative trees. Tri-axial accelerometers packed in a small titanium housing were used. Trees were assessed before and after the re-shaping pruning. This study reports the first data about the dynamic behavior of centuries-old tree skeletons, in the context of very large-sized olive trees, while taking into account the effects of two different vibrations application modes: a realistic one represented by the system vibration head-tree, originated when the gripper of a shaking machine wrapped and fastened the main branch of the olive trees, and a more speculative one, represented by a single impulse of a short-duration force originated by a hammer.ResultsAfter pruning, spectral density increased 10 fold in the tertiary branches of pruned trees (ranging 1.0-10 m s-2) compared to that of not-pruned ones (ranging 0.1-1.0 m s-2) at frequency >50 Hz under vibration excitation. Moreover, vibrational decay times (120-150 ms) and amplitude (>10-1 m s-2) were higher under single-impulse excitation.DiscussionA more rational pruning applied to ancient large-sized olive trees significantly increased the vibration transmission under both impulse and vibratory excitation forces, without affected their typical "look". Moreover, these insights are helpful in turn in achieving maximum fruit-removal efficiency. These insights could be applied to various horticultural conditions which would improve the economic sustainability of monumental olive trees, a key portion of the Mediterranean landscape and cultural heritage.

Project description:BackgroundOlive tree (Olea europaea L. subsp. europaea, Oleaceae) has been the most emblematic perennial crop for Mediterranean countries since its domestication around 6000 years ago in the Levant. Two taxonomic varieties are currently recognized: cultivated (var. europaea) and wild (var. sylvestris) trees. However, it remains unclear whether olive cultivars derive from a single initial domestication event followed by secondary diversification, or whether cultivated lineages are the result of more than a single, independent primary domestication event. To shed light into the recent evolution and domestication of the olive tree, here we analyze a group of newly sequenced and available genomes using a phylogenomics and population genomics framework.ResultsWe improved the assembly and annotation of the reference genome, newly sequenced the genomes of twelve individuals: ten var. europaea, one var. sylvestris, and one outgroup taxon (subsp. cuspidata)-and assembled a dataset comprising whole genome data from 46 var. europaea and 10 var. sylvestris. Phylogenomic and population structure analyses support a continuous process of olive tree domestication, involving a major domestication event, followed by recurrent independent genetic admixture events with wild populations across the Mediterranean Basin. Cultivated olives exhibit only slightly lower levels of genetic diversity than wild forms, which can be partially explained by the occurrence of a mild population bottleneck 3000-14,000 years ago during the primary domestication period, followed by recurrent introgression from wild populations. Genes associated with stress response and developmental processes were positively selected in cultivars, but we did not find evidence that genes involved in fruit size or oil content were under positive selection. This suggests that complex selective processes other than directional selection of a few genes are in place.ConclusionsAltogether, our results suggest that a primary domestication area in the eastern Mediterranean basin was followed by numerous secondary events across most countries of southern Europe and northern Africa, often involving genetic admixture with genetically rich wild populations, particularly from the western Mediterranean Basin.

Project description:BackgroundUnderstanding the evolution of cultivated barley is important for two reasons. First, the evolutionary relationships between different landraces might provide information on the spread and subsequent development of barley cultivation, including the adaptation of the crop to new environments and its response to human selection. Second, evolutionary information would enable landraces with similar traits but different genetic backgrounds to be identified, providing alternative strategies for the introduction of these traits into modern germplasm.ResultsThe evolutionary relationships between 651 barley landraces were inferred from the genotypes for 24 microsatellites. The landraces could be divided into nine populations, each with a different geographical distribution. Comparisons with ear row number, caryopsis structure, seasonal growth habit and flowering time revealed a degree of association between population structure and phenotype, and analysis of climate variables indicated that the landraces are adapted, at least to some extent, to their environment. Human selection and/or environmental adaptation may therefore have played a role in the origin and/or maintenance of one or more of the barley landrace populations. There was also evidence that at least some of the population structure derived from geographical partitioning set up during the initial spread of barley cultivation into Europe, or reflected the later introduction of novel varieties. In particular, three closely-related populations were made up almost entirely of plants with the daylength nonresponsive version of the photoperiod response gene PPD-H1, conferring adaptation to the long annual growth season of northern Europe. These three populations probably originated in the eastern Fertile Crescent and entered Europe after the initial spread of agriculture.ConclusionsThe discovery of population structure, combined with knowledge of associated phenotypes and environmental adaptations, enables a rational approach to identification of landraces that might be used as sources of germplasm for breeding programs. The population structure also enables hypotheses concerning the prehistoric spread and development of agriculture to be addressed.

Dataset Information

A comparative analysis of genetic variation in rootstocks and scions of old olive trees - a window into the history of olive cultivation practices and past genetic variation.

Publications

A comparative analysis of genetic variation in rootstocks and scions of old olive trees - a window into the history of olive cultivation practices and past genetic variation.

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