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ULK1 translocates to mitochondria and phosphorylates FUNDC1 to regulate mitophagy.

ABSTRACT: Autophagy eliminates dysfunctional mitochondria in an intricate process known as mitophagy. ULK1 is critical for the induction of autophagy, but its substrate(s) and mechanism of action in mitophagy remain unclear. Here, we show that ULK1 is upregulated and translocates to fragmented mitochondria upon mitophagy induction by either hypoxia or mitochondrial uncouplers. At mitochondria, ULK1 interacts with FUNDC1, phosphorylating it at serine 17, which enhances FUNDC1 binding to LC3. A ULK1-binding-deficient mutant of FUNDC1 prevents ULK1 translocation to mitochondria and inhibits mitophagy. Finally, kinase-active ULK1 and a phospho-mimicking mutant of FUNDC1 rescue mitophagy in ULK1-null cells. Thus, we conclude that FUNDC1 regulates ULK1 recruitment to damaged mitochondria, where FUNDC1 phosphorylation by ULK1 is crucial for mitophagy.

SUBMITTER: Wu W

PROVIDER: S-EPMC4210082 | biostudies-other | 2014 May

REPOSITORIES: biostudies-other

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ULK1 translocates to mitochondria and phosphorylates FUNDC1 to regulate mitophagy.

Wu Wenxian W Tian Weili W Hu Zhe Z Chen Guo G Huang Lei L Li Wen W Zhang Xingli X Xue Peng P Zhou Changqian C Liu Lei L Zhu Yushan Y Zhang Xingliang X Li Longxuan L Zhang Liangqing L Sui Senfang S Zhao Bin B Feng Du D

EMBO reports 20140326 5

Autophagy eliminates dysfunctional mitochondria in an intricate process known as mitophagy. ULK1 is critical for the induction of autophagy, but its substrate(s) and mechanism of action in mitophagy remain unclear. Here, we show that ULK1 is upregulated and translocates to fragmented mitochondria upon mitophagy induction by either hypoxia or mitochondrial uncouplers. At mitochondria, ULK1 interacts with FUNDC1, phosphorylating it at serine 17, which enhances FUNDC1 binding to LC3. A ULK1-binding ...[more]

PMID: 24671035

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