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MicroRNA-9 controls dendritic development by targeting REST.

ABSTRACT: MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner. Using this novel approach, we found that miR-9 controls dendritic growth and synaptic transmission in vivo. Furthermore, we demonstrate that miR-9-mediated downregulation of the transcriptional repressor REST is essential for proper dendritic growth.

SUBMITTER: Giusti SA

PROVIDER: S-EPMC4235007 | biostudies-other | 2014

REPOSITORIES: biostudies-other

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MicroRNA-9 controls dendritic development by targeting REST.

Giusti Sebastian A SA Vogl Annette M AM Brockmann Marisa M MM Vercelli Claudia A CA Rein Martin L ML Trümbach Dietrich D Wurst Wolfgang W Cazalla Demian D Stein Valentin V Deussing Jan M JM Refojo Damian D

eLife 20141118

MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manne ...[more]

PMID: 25406064

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