Ontology highlight
ABSTRACT:
SUBMITTER: Du D
PROVIDER: S-EPMC4349145 | biostudies-other | 2015 Mar
REPOSITORIES: biostudies-other
Du Deyao D Wang Lu L Tian Yuqing Y Liu Hao H Tan Huarong H Niu Guoqing G
Scientific reports 20150304
Several strategies have been used to clone large DNA fragments directly from bacterial genome. Most of these approaches are based on different site-specific recombination systems consisting of a specialized recombinase and its target sites. In this study, a novel strategy based on phage ϕBT1 integrase-mediated site-specific recombination was developed, and used for simultaneous Streptomyces genome engineering and cloning of antibiotic gene clusters. This method has been proved successful for the ...[more]