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Effects of α-enolase (ENO1) over-expression on malignant biological behaviors of AGS cells.


ABSTRACT: OBJECTIVE: To investigate the effects of α-Enolase (ENO1) over-expression on the proliferative and migratory abilities of AGS cells. METHODS: The target gene was cloned and mounted to the eukaryotic expression vector pcDNA3.1(+), then was transfected into gastric cancer cell lines AGS. mRNA and protein level of ENO1 in AGS cells were verified by real-time quantitative RT-PCR and Western Blot, respectively. The effects of over-expression of ENO1 on proliferative and migratory abilities of AGS cells were detected by the experiments of CCK-8, colony formation and wound healing assays. RESULTS: The eukaryotic expression vector pcDNA3.1(+)/eno1 was successfully constructed, and verified by sequencing. It was shown from the cell proliferation curves that the proliferative ability of AGS-ENO1 transfected group was higher than that of the control group after 72 hours (t = 3.44, P = 0.04), meanwhile, the number of the cell-colonies of the AGS-ENO1 group were significantly greater than that of the control group (t = 5.26, P = 0.01). For the ability of migration, it was significantly enhanced in the over-expression ENO1 cells than in the negative cells (t = 7.35, P < 0.001). CONCLUSION: The over-expression of ENO1 protein can enhance the abilities of proliferation and migration in gastric cancer cells of AGS, which indicates that ENO1 may be an important potential tumor-marker associated with the development of gastric cancer.

SUBMITTER: Liu YQ 

PROVIDER: S-EPMC4358447 | biostudies-other | 2015

REPOSITORIES: biostudies-other

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Effects of α-enolase (ENO1) over-expression on malignant biological behaviors of AGS cells.

Liu Yang-Qi YQ   Huang Zhi-Gang ZG   Li Ge-Nan GN   Du Jin-Lin JL   Ou Yang-Ping YP   Zhang Xiang-Ning XN   Chen Ting-Ting TT   Liang Qi-Lian QL  

International journal of clinical and experimental medicine 20150115 1


<h4>Objective</h4>To investigate the effects of α-Enolase (ENO1) over-expression on the proliferative and migratory abilities of AGS cells.<h4>Methods</h4>The target gene was cloned and mounted to the eukaryotic expression vector pcDNA3.1(+), then was transfected into gastric cancer cell lines AGS. mRNA and protein level of ENO1 in AGS cells were verified by real-time quantitative RT-PCR and Western Blot, respectively. The effects of over-expression of ENO1 on proliferative and migratory abiliti  ...[more]

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