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Another member of the cyclic nucleotide-gated channel family, expressed in testis, kidney, and heart.

ABSTRACT: Cyclic nucleotide-gated cation channels are essential in visual and olfactory signal transduction. An additional member of the cGMP-gated channel family, termed CNG-3, has been cloned from bovine kidney. Its deduced amino acid sequence is 60% and 62% identical with the CNG-channel proteins from bovine rod outer segment and bovine olfactory epithelium, respectively. Northern analysis and sequences amplified by the PCR showed that the CNG-3 mRNA is present in testis, kidney, and heart. Calcium permeated the expressed channel in the presence of extracellular Mg2+ and Na+ at membrane potentials from -100 to +45 mV. It is likely that CNG-3 protein is responsible for cGMP-induced Ca2+ entry in cells other than sensory cells.

SUBMITTER: Biel M

PROVIDER: S-EPMC43608 | biostudies-other | 1994 Apr

REPOSITORIES: biostudies-other

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Another member of the cyclic nucleotide-gated channel family, expressed in testis, kidney, and heart.

Biel M M Zong X X Distler M M Bosse E E Klugbauer N N Murakami M M Flockerzi V V Hofmann F F

Proceedings of the National Academy of Sciences of the United States of America 19940401 9

Cyclic nucleotide-gated cation channels are essential in visual and olfactory signal transduction. An additional member of the cGMP-gated channel family, termed CNG-3, has been cloned from bovine kidney. Its deduced amino acid sequence is 60% and 62% identical with the CNG-channel proteins from bovine rod outer segment and bovine olfactory epithelium, respectively. Northern analysis and sequences amplified by the PCR showed that the CNG-3 mRNA is present in testis, kidney, and heart. Calcium per ...[more]

PMID: 8170936

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