Ontology highlight
ABSTRACT:
SUBMITTER: Cuculis L
PROVIDER: S-EPMC4458887 | biostudies-other | 2015 Jun
REPOSITORIES: biostudies-other
Cuculis Luke L Abil Zhanar Z Zhao Huimin H Schroeder Charles M CM
Nature communications 20150601
Transcription activator-like effector (TALE) proteins are a class of programmable DNA-binding proteins for which the fundamental mechanisms governing the search process are not fully understood. Here we use single-molecule techniques to directly observe TALE search dynamics along DNA templates. We find that TALE proteins are capable of rapid diffusion along DNA using a combination of sliding and hopping behaviour, which suggests that the TALE search process is governed in part by facilitated dif ...[more]