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Label-free imaging to study phenotypic behavioural traits of cells in complex co-cultures.

ABSTRACT: Time-lapse imaging is a fundamental tool for studying cellular behaviours, however studies of primary cells in complex co-culture environments often requires fluorescent labelling and significant light exposure that can perturb their natural function over time. Here, we describe ptychographic phase imaging that permits prolonged label-free time-lapse imaging of microglia in the presence of neurons and astrocytes, which better resembles in vivo microenvironments. We demonstrate the use of ptychography as an assay to study the phenotypic behaviour of microglial cells in primary neuronal co-cultures through the addition of cyclosporine A, a potent immune-modulator.

SUBMITTER: Suman R

PROVIDER: S-EPMC4768090 | biostudies-other | 2016 Feb

REPOSITORIES: biostudies-other

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Label-free imaging to study phenotypic behavioural traits of cells in complex co-cultures.

Suman Rakesh R Smith Gabrielle G Hazel Kathryn E A KE Kasprowicz Richard R Coles Mark M O'Toole Peter P Chawla Sangeeta S

Scientific reports 20160226

Time-lapse imaging is a fundamental tool for studying cellular behaviours, however studies of primary cells in complex co-culture environments often requires fluorescent labelling and significant light exposure that can perturb their natural function over time. Here, we describe ptychographic phase imaging that permits prolonged label-free time-lapse imaging of microglia in the presence of neurons and astrocytes, which better resembles in vivo microenvironments. We demonstrate the use of ptychog ...[more]

PMID: 26915695

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