Ontology highlight
ABSTRACT:
SUBMITTER: Peters DT
PROVIDER: S-EPMC4909860 | biostudies-other | 2016 May
REPOSITORIES: biostudies-other
Peters Derek T DT Henderson Christopher A CA Warren Curtis R CR Friesen Max M Xia Fang F Becker Caroline E CE Musunuru Kiran K Cowan Chad A CA
Development (Cambridge, England) 20160501 9
Hepatocyte-like cells (HLCs) are derived from human pluripotent stem cells (hPSCs) in vitro, but differentiation protocols commonly give rise to a heterogeneous mixture of cells. This variability confounds the evaluation of in vitro functional assays performed using HLCs. Increased differentiation efficiency and more accurate approximation of the in vivo hepatocyte gene expression profile would improve the utility of hPSCs. Towards this goal, we demonstrate the purification of a subpopulation of ...[more]