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SIRT1, 2, 3 protect mouse oocytes from postovulatory aging.

ABSTRACT: The quality of metaphase II oocytes will undergo a time-dependent deterioration following ovulation as the result of the oocyte aging process. In this study, we determined that the expression of sirtuin family members (SIRT1, 2, 3) was dramatically reduced in mouse oocytes aged in vivo or in vitro. Increased intracellular ROS was observed when SIRT1, 2, 3 activity was inhibited. Increased frequency of spindle defects and disturbed distribution of mitochondria were also observed in MII oocytes aged in vitro after treatment with Nicotinamide (NAM), indicating that inhibition of SIRT1, 2, 3 may accelerate postovulatory oocyte aging. Interestingly, when MII oocytes were exposed to caffeine, the decline of SIRT1, 2, 3 mRNA levels was delayed and the aging-associated defective phenotypes could be improved. The results suggest that the SIRT1, 2, 3 pathway may play a potential protective role against postovulatory oocyte aging by controlling ROS generation.

SUBMITTER: Zhang T

PROVIDER: S-EPMC4925822 | biostudies-other | 2016 Apr

REPOSITORIES: biostudies-other

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SIRT1, 2, 3 protect mouse oocytes from postovulatory aging.

Zhang Teng T Zhou Yang Y Li Li L Wang Hong-Hui HH Ma Xue-Shan XS Qian Wei-Ping WP Shen Wei W Schatten Heide H Sun Qing-Yuan QY

Aging 20160401 4

The quality of metaphase II oocytes will undergo a time-dependent deterioration following ovulation as the result of the oocyte aging process. In this study, we determined that the expression of sirtuin family members (SIRT1, 2, 3) was dramatically reduced in mouse oocytes aged in vivo or in vitro. Increased intracellular ROS was observed when SIRT1, 2, 3 activity was inhibited. Increased frequency of spindle defects and disturbed distribution of mitochondria were also observed in MII oocytes ag ...[more]

PMID: 26974211

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