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Expression cloning of the murine and human interleukin 9 receptor cDNAs.

ABSTRACT: Interleukin 9 (IL-9) is a T-cell-derived lymphokine that induces the proliferation of various lymphoid and hemopoietic cells. A cDNA clone encoding the murine IL-9 receptor was isolated by expression cloning in COS cells and screening with 125I-labeled IL-9. Transient expression of this cDNA produced high-affinity binding sites for IL-9. The predicted 52-kDa protein contains a putative signal peptide and a typical transmembrane domain. A cDNA for the human homologue was isolated by cross-hybridization. Transfection of this cDNA in a murine T-cell clone conferred responsiveness to human IL-9. Sequence analysis revealed that the IL-9 receptor belongs to the recently described hematopoietin receptor super-family and is expressed in membrane-bound and soluble forms.

SUBMITTER: Renauld JC

PROVIDER: S-EPMC49358 | biostudies-other | 1992 Jun

REPOSITORIES: biostudies-other

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Expression cloning of the murine and human interleukin 9 receptor cDNAs.

Renauld J C JC Druez C C Kermouni A A Houssiau F F Uyttenhove C C Van Roost E E Van Snick J J

Proceedings of the National Academy of Sciences of the United States of America 19920601 12

Interleukin 9 (IL-9) is a T-cell-derived lymphokine that induces the proliferation of various lymphoid and hemopoietic cells. A cDNA clone encoding the murine IL-9 receptor was isolated by expression cloning in COS cells and screening with 125I-labeled IL-9. Transient expression of this cDNA produced high-affinity binding sites for IL-9. The predicted 52-kDa protein contains a putative signal peptide and a typical transmembrane domain. A cDNA for the human homologue was isolated by cross-hybridi ...[more]

PMID: 1376929

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