Unknown

Dataset Information

0

Calcium channels from Cyprinus carpio skeletal muscle.


ABSTRACT: The complete amino acid sequence of the L-type calcium channel alpha 1 subunit from the carp (Cyprinus carpio) white skeletal muscle was deduced by cDNA cloning and sequence analysis. The open reading frame encodes 1852 amino acids (Mr 210,060). A 155-amino acid COOH-terminal sequence (after the fourth internal repeat) is evolutionarily preserved (90% homology) and may represent an important functional domain of L-type calcium channels. The photolabeled, membrane-bound, and purified carp alpha 1 subunits have masses of 211 and 190 kDa. The purified channel could not be phosphorylated by cAMP-dependent protein kinase. Two glycoproteins (alpha 2 subunits) are associated with the alpha 1 subunit and change their apparent masses from 235 and 220 kDa to 159 kDa upon reduction of disulfide bonds. Nucleic acid hybridization with alpha 2 cDNA revealed an 8.0-kilobase transcript in carp skeletal muscle. Evidence for a copurification of subunits similar in size to mammalian beta or gamma subunits was not obtained.

SUBMITTER: Grabner M 

PROVIDER: S-EPMC50886 | biostudies-other | 1991 Feb

REPOSITORIES: biostudies-other

altmetric image

Publications

Calcium channels from Cyprinus carpio skeletal muscle.

Grabner M M   Friedrich K K   Knaus H G HG   Striessnig J J   Scheffauer F F   Staudinger R R   Koch W J WJ   Schwartz A A   Glossmann H H  

Proceedings of the National Academy of Sciences of the United States of America 19910201 3


The complete amino acid sequence of the L-type calcium channel alpha 1 subunit from the carp (Cyprinus carpio) white skeletal muscle was deduced by cDNA cloning and sequence analysis. The open reading frame encodes 1852 amino acids (Mr 210,060). A 155-amino acid COOH-terminal sequence (after the fourth internal repeat) is evolutionarily preserved (90% homology) and may represent an important functional domain of L-type calcium channels. The photolabeled, membrane-bound, and purified carp alpha 1  ...[more]

Similar Datasets

2008-08-15 | E-GEOD-10980 | biostudies-arrayexpress
2008-08-15 | GSE10980 | GEO
| S-EPMC7800765 | biostudies-literature
2019-01-15 | GSE125039 | GEO
2018-09-25 | GSE73429 | GEO
| S-EPMC7692175 | biostudies-literature
| PRJNA260658 | ENA
| PRJNA493170 | ENA
| S-EPMC1750873 | biostudies-literature
| S-EPMC4709859 | biostudies-literature