Latent pH-responsive ratiometric fluorescent cluster based on self-assembled photoactivated SNARF derivatives.

ABSTRACT: We have developed a self-assembled fluorescent cluster comprising a seminaphthorhodafluor (SNARF) derivative protected by a photoremovable o-nitrobenzyl group. Prior to UV irradiation, a colorless and nonfluorescent cluster was spontaneously assembled in aqueous solution. After UV irradiation, the self-assembled cluster remained intact and showed a large enhancement in pH-responsive fluorescence. The unique pH responsive fluorescent cluster could be used as a dual-emissive ratiometric fluorescent pH probe not only in the test tube but also in HeLa cell cultures.

SUBMITTER: Nakata E 

PROVIDER: S-EPMC5101900 | biostudies-other | 2016

REPOSITORIES: biostudies-other