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Complete sequence of human vinculin and assignment of the gene to chromosome 10.

ABSTRACT: We have determined the complete sequence of human vinculin, a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Comparison of human and chicken embryo vinculin sequences shows that both proteins contain 1066 amino acids and exhibit a high level of sequence identity (greater than 95%). The region of greatest divergence falls within three 112-amino acid repeats spanning residues 259-589. Interestingly, nematode vinculin lacks one of these central repeats. The regions of human vinculin that are N- and C-terminal to the repeats show 54% and 61% sequence identity, respectively, to nematode vinculin. Southern blots of human genomic DNA hybridized with short vinculin cDNA fragments indicate that there is a single vinculin gene. By using a panel of human-rodent somatic cell hybrids, the human vinculin gene was mapped to chromosome 10q11.2-qter.

SUBMITTER: Weller PA

PROVIDER: S-EPMC54388 | biostudies-other | 1990 Aug

REPOSITORIES: biostudies-other

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Complete sequence of human vinculin and assignment of the gene to chromosome 10.

Weller P A PA Ogryzko E P EP Corben E B EB Zhidkova N I NI Patel B B Price G J GJ Spurr N K NK Koteliansky V E VE Critchley D R DR

Proceedings of the National Academy of Sciences of the United States of America 19900801 15

We have determined the complete sequence of human vinculin, a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Comparison of human and chicken embryo vinculin sequences shows that both proteins contain 1066 amino acids and exhibit a high level of sequence identity (greater than 95%). The region of greatest divergence falls within three 112-amino acid repeats spanning residues 259-589. Interestingly, nematode vinculin lacks one of these central repeats. The regions of hum ...[more]

PMID: 2116004

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