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Microstructure and Characteristic of BiVO₄ Prepared under Different pH Values: Photocatalytic Efficiency and Antibacterial Activity.

ABSTRACT: In the present study, BiVO₄ sample was prepared under different pH 0.5-13 without capping agent. Different morphology characteristics were observed, such as sheet crystal structure, cross crystal structure and branching crystal structure. The mechanism of the formation of BiVO₄ nanostructure was discussed. Under acid condition, sheet crystal structure was obtained. The phenomenon could be attributed to polymerization of vanadate in the presence of H⁺. In the weak alkaline solution, across structure and branching type morphology was obtained. The photocatalytic efficiency for the samples ranked as pH 5 > pH 3 > pH 7 > pH 9 > pH 1 > pH 11 > pH 13 > blank, which is in good agreement with X-ray diffraction (XRD) result. E. coli envelop was damaged in the presence of BiVO₄ under visible light. The protrusion on envelop was diminished by BiVO₄. Attenuated Total Reflection Fourier transformed Infrared Spectroscopy (ATR-FTIR) results suggested the intensity was weakened for the amide, phosphoric, -COO- group and C-H bond in lipopolysaccharides (LPS), peptidoglycan and periplasm molecules.

SUBMITTER: Qu Z

PROVIDER: S-EPMC5456712 | biostudies-other | 2016 Feb

REPOSITORIES: biostudies-other

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Exceptional antibacterial and cytotoxic potency of monodisperse greener AgNPs prepared under optimized pH and temperature.

Project description:In the present work, silver nanoparticles were prepared by using the extract of Camellia Sinensis. The extract contains phytochemicals which are mainly polyphenols acting as the natural reducing and stabilizing agents leading to the formation of uniformly dispersed and stabilized silver nanoparticles. The synthesis of silver nanoparticles was significantly influenced by the impact of the pH, as well as temperature conditions. It was found that at pH 5 and 25 °C, nanoparticles of different morphologies (spherical, polygonal, capsule) and sizes were formed. However, with the increase in temperature from 25 °C to 65 °C but at the same pH, these particles started attaining the spherical shape of different sizes owing to an increase in the reduction rate. Furthermore, for the reaction of the mixture at 65 °C, an increase in pH from 5 to 11 led to an increase in the monodispersity of spherically shaped nanoparticles, attributed to the hydroxide ions facilitated reduction. The prepared nanoparticles were investigated for their antibacterial activity using Nathan's Agar Well-Diffusion method. It was found that AgNPs prepared at pH 9 and 65 °C demonstrated strong antibacterial activity against gram-negative Escherichia coli in contrast to gram-positive Staphylococcus aureus. In reference to the cytotoxic potency, the prepared AgNPs showed clear cytotoxicity for HeLa cells and showcased a close relationship between activity and concentration as evidenced by the decrease in the percentage (100 to 30%) of metabolically active cells up to 25 µM-75 µM concentration of silver nanoparticles.

| S-EPMC7858571 | biostudies-literature

Proteome of Corynebacterium glutamicum at different pH values

Project description:Recently, we established Corynebacterium glutamicum as a suitable production host for various bacteriocins including garvicin Q (GarQ). Here, we establish secretion of GarQ by C. glutamicum via the Sec translocon. At neutral pH, the cationic peptide is efficiently adsorbed to the negatively charged envelope of producer bacteria limiting availability of the bacteriocin in culture supernatants. Using a reporter strain and proteomic analyses, we identified HtrA, a protease associated with secretion stress, as another potential factor limiting GarQ production.

2024-01-08 | PXD046499 | Pride

Acute Toxicity and Hazardous Concentrations of Zinc to Native Freshwater Organisms Under Different pH Values in China.

Project description:Zinc bioavailability to aquatic organisms varies greatly under different pH values. In the present study, five native species in China and four common international test species were selected to investigate the influence of changing pH on acute zinc toxicity. The results showed that the higher trophic levels exhibited increasing sensitivity to zinc as pH decreased. However, when the pH value was between 8 and 11, the acute toxicity of zinc was relatively constant. In addition, by using a species-sensitivity distribution (SSD) method, the short-term hazardous concentrations of zinc at different pH values (based on the 5th percentiles of the pH-specific SSDs) were determined to be 17.26 µg/L (pH 4), 48.31 µg/L (pH 5), 80.34 µg/L (pH 6) and 230.6 µg/L (pH 7), respectively. The present study provides useful information for deriving water quality criteria and assessing the risks of metals in the near future.

| S-EPMC6647607 | biostudies-literature

ZnO Nanorods with High Photocatalytic and Antibacterial Activity under Solar Light Irradiation.

Project description:ZnO nanorods (NRs) with an average length and diameter of 186 and 20 nm, respectively, were prepared through a mild solvothermal route and used as photocatalysts either as dispersed powder or immobilized on glass slides. The ZnO NRs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). Dispersed ZnO NRs and, to a lesser extent, immobilized ZnO NRs were demonstrated to exhibit high photocatalytic activity under simulated sunlight of low intensity (5.5 mW/cm²) both for the degradation of the Orange II dye and for Escherichia coli bacterial decontamination (2.5-fold survival decrease after 180 min irradiation for immobilized NRs). SEM, atomic force microscopy (AFM), fluorescence spectroscopy, and epifluorescence microscopy demonstrate that cell surface damages are responsible of bacterial inactivation. The immobilized ZnO NRs could be reused up to five times for bacterial decontamination at comparable efficiency and therefore have great potential for real environmental applications.

| S-EPMC6266891 | biostudies-literature

Transcription profiling of Corynebacterium glutamicum grown in fermenter at different pH values.

Project description:Analysis of the transcriptomes of cell grown at different pH levels pH6.0, pH7.5, pH9.0; Cells were grown in a fermenter at different pH values to an oD600 between 4.5 to 11.5

2009-10-22 | E-MTAB-151 | biostudies-arrayexpress

Antibacterial Activity and Protection Efficiency of Polyvinyl Butyral Nanofibrous Membrane Containing Thymol Prepared through Vertical Electrospinning.

Project description:Human safety, health management, and disease transmission prevention have become crucial tasks in the present COVID-19 pandemic situation. Masks are widely available and create a safer and disease transmission-free environment. This study presents a facile method of fabricating masks through electrospinning nontoxic polyvinyl butyral (PVB) polymeric matrix with the antibacterial component Thymol, a natural phenol monoterpene. Based on the results of Japanese Industrial Standards and American Association of Textile Chemists and Colorists methods, the maximum antibacterial value of the mask against Gram-positive and Gram-negative bacteria was 5.6 and 6.4, respectively. Moreover, vertical electrospinning was performed to prepare Thymol/PVB nanofiber masks, and the effects of parameters on the submicron particulate filtration efficiency (PFE), differential pressure, and bacterial filtration efficiency (BFE) were determined. Thorough optimization of the small-diameter nanofiber-based antibacterial mask led to denser accumulation and improved PFE and pressure difference; the mask was thus noted to meet the present pandemic requirements. The as-developed nanofibrous masks have the antibacterial activity suggested by the National Standard of the Republic of China (CNS 14774) for general medical masks. Their BFE reaches 99.4%, with a pressure difference of <5 mmH2O/cm2. The mask can safeguard human health and promote a healthy environment.

| S-EPMC8036783 | biostudies-literature

Expression analysis of the fungus Ustilago maydis WT strain FB2 (a2b2) at different pH values

Project description:Elicidate the changes of the transcriptional program response of U. maydis WT strain (a2b2) when transfered from neutrality to acidic or alkaline medium The mutants strains CL211 and GP25 used as controls as well as the WT strain are described in Martínez-Espinoza et al., 1997; 2004;González-Prieto et al., 2014 and Banuett & Herskowitz, 1989 respectively.

2016-02-23 | GSE75053 | GEO

Degradation Kinetics and Shelf Life of N-acetylneuraminic Acid at Different pH Values.

Project description:The objective of this study was to investigate the stability and degradation kinetics of N-acetylneuraminic acid (Neu5Ac). The pH of the solution strongly influenced the stability of Neu5Ac, which was more stable at neutral pH and low temperatures. Here, we provide detailed information on the degradation kinetics of Neu5Ac at different pH values (1.0, 2.0, 11.0 and 12.0) and temperatures (60, 70, 80 and 90 °C). The study of the degradation of Neu5Ac under strongly acidic conditions (pH 1.0-2.0) is highly pertinent for the hydrolysis of polysialic acid. The degradation kinetics of alkaline deacetylation were also studied. Neu5Ac was highly stable at pH 3.0-10.0, even at high temperature, but the addition of H2O2 greatly reduced its stability at pH 5.0, 7.0 and 9.0. Although Neu5Ac has a number of applications in products of everyday life, there are no reports of rigorous shelf-life studies. This research provides kinetic data that can be used to predict product shelf lives at different temperatures and pH values.

| S-EPMC7663827 | biostudies-literature

Cytotoxicity Evaluation of Minimum Antibacterial Values of Different Medicaments Used in Endodontic Regenerative Procedures.

Project description:OBJECTIVES:?This study aimed to evaluate the cytotoxicity of minimum antibacterial values of medicaments used in endodontic regeneration on stem cells. MATERIALS AND METHODS:?"Minimum inhibitory concentration," "minimum bactericidal concentration," and "minimum biofilm inhibitory concentration" of triple and double antibiotic paste, a modified triple antibiotic paste (minocycline replaced by clindamycin), Augmentin, and calcium hydroxide were determined using Enterococcus faecalis (ATCC 29212) by microtiter plate method. Direct cytotoxic effects of drugs were evaluated by lactate dehydrogenase and water-soluble tetrazolium salt-1 assays using stem cells of apical papilla obtained from immature third molars via enzymatic digestion. STATISTICAL ANALYSIS:?Data were analyzed using IBM SPSS Statistics 24, one-way analysis of variance and post hoc comparisons. The statistical power was set at p < 0.05. RESULTS:?All medicaments caused similar cytotoxicity and cell proliferation at "minimum inhibitory concentration" (p > 0.05) except Augmentin which was significantly more toxic than others (p < 0.05). At "minimum bactericidal concentration," calcium hydroxide was more toxic than other drugs (p < 0.001), but its adverse effect on cell proliferation was the same as Augmentin (p > 0.05). Triple and double antibiotic paste revealed similar favorable effects in terms of toxicity and proliferation rate at most of the tested concentrations (p > 0.05). At "minimum biofilm inhibitory concentration" both the modified paste and Augmentin caused less proliferation rate than triple and double antibiotic paste (p < 0.001and p < 0.05, respectively) and Augmentin induced more cytotoxicity (p < 0.05). CONCLUSIONS:?Considering the antimicrobial potency, triple antibiotic paste seems to be the safest drug for the stem cells of apical papilla, while Augmentin may have some adverse effects.

| S-EPMC6938419 | biostudies-literature

Synergistic Effect of Fluorinated and N Doped TiO? Nanoparticles Leading to Different Microstructure and Enhanced Photocatalytic Bacterial Inactivation.

Project description:This work focuses on the development of a facile and scalable wet milling method followed by heat treatment to prepare fluorinated and/or N-doped TiO? nanopowders with improved photocatalytic properties under visible light. The structural and electronic properties of doped particles were investigated by various techniques. The successful doping of TiO? was confirmed by X-ray photoelectron spectroscopy (XPS), and the atoms appeared to be mainly located in interstitial positions for N whereas the fluorination is located at the TiO? surface. The formation of intragap states was found to be responsible for the band gap narrowing leading to the faster bacterial inactivation dynamics observed for the fluorinated and N doped TiO? particles compared to N-doped TiO?. This was attributed to a synergistic effect. The results presented in this study confirmed the suitability of the preparation approach for the large-scale production of cost-efficient doped TiO? for effective bacterial inactivation.

| S-EPMC5707608 | biostudies-literature

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