Unknown

Dataset Information

0

In Vivo Osteogenic Differentiation of Human Embryoid Bodies in an Injectable in Situ-Forming Hydrogel.


ABSTRACT: In this study, we examined the in vivo osteogenic differentiation of human embryoid bodies (hEBs) by using an injectable in situ-forming hydrogel. A solution containing MPEG-b-(polycaprolactone-ran-polylactide) (MCL) and hEBs was easily prepared at room temperature. The MCL solution with hEBs and osteogenic factors was injected into nude mice and developed into in situ-forming hydrogels at the injection sites; these hydrogels maintained their shape even after 12 weeks in vivo, thereby indicating that the in situ-forming MCL hydrogel was a suitable scaffold for hEBs. The in vivo osteogenic differentiation was observed only in the in situ gel-forming MCL hydrogel in the presence of hEBs and osteogenic factors. In conclusion, this preliminary study suggests that hEBs and osteogenic factors embedded in an in situ-forming MCL hydrogel may provide numerous benefits as a noninvasive alternative for allogeneic tissue engineering applications.

SUBMITTER: Kim DY 

PROVIDER: S-EPMC5521290 | biostudies-other | 2013 Jul

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC5529463 | biostudies-literature
| S-EPMC7345654 | biostudies-literature
| S-EPMC10535719 | biostudies-literature
| S-EPMC4426716 | biostudies-literature
| S-EPMC5750467 | biostudies-literature
| S-EPMC3013227 | biostudies-literature
| S-EPMC4340738 | biostudies-literature
| S-EPMC8865040 | biostudies-literature
| S-EPMC4157362 | biostudies-literature
| S-EPMC2567159 | biostudies-other