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A novel region in the CaV2.1 ?1 subunit C-terminus regulates fast synaptic vesicle fusion and vesicle docking at the mammalian presynaptic active zone.


ABSTRACT: In central nervous system (CNS) synapses, action potential-evoked neurotransmitter release is principally mediated by CaV2.1 calcium channels (CaV2.1) and is highly dependent on the physical distance between CaV2.1 and synaptic vesicles (coupling). Although various active zone proteins are proposed to control coupling and abundance of CaV2.1 through direct interactions with the CaV2.1 ?1 subunit C-terminus at the active zone, the role of these interaction partners is controversial. To define the intrinsic motifs that regulate coupling, we expressed mutant CaV2.1 ?1 subunits on a CaV2.1 null background at the calyx of Held presynaptic terminal. Our results identified a region that directly controlled fast synaptic vesicle release and vesicle docking at the active zone independent of CaV2.1 abundance. In addition, proposed individual direct interactions with active zone proteins are insufficient for CaV2.1 abundance and coupling. Therefore, our work advances our molecular understanding of CaV2.1 regulation of neurotransmitter release in mammalian CNS synapses.

SUBMITTER: Lubbert M 

PROVIDER: S-EPMC5548488 | biostudies-other | 2017 Aug

REPOSITORIES: biostudies-other

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A novel region in the Ca<sub>V</sub>2.1 α<sub>1</sub> subunit C-terminus regulates fast synaptic vesicle fusion and vesicle docking at the mammalian presynaptic active zone.

Lübbert Matthias M   Goral R Oliver RO   Satterfield Rachel R   Putzke Travis T   van den Maagdenberg Arn Mjm AM   Kamasawa Naomi N   Young Samuel M SM  

eLife 20170808


In central nervous system (CNS) synapses, action potential-evoked neurotransmitter release is principally mediated by Ca<sub>V</sub>2.1 calcium channels (Ca<sub>V</sub>2.1) and is highly dependent on the physical distance between Ca<sub>V</sub>2.1 and synaptic vesicles (coupling). Although various active zone proteins are proposed to control coupling and abundance of Ca<sub>V</sub>2.1 through direct interactions with the Ca<sub>V</sub>2.1 α1 subunit C-terminus at the active zone, the role of the  ...[more]

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