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Bleomycin Can Cleave an Oncogenic Noncoding RNA.

ABSTRACT: Noncoding RNAs are pervasive in cells and contribute to diseases such as cancer. A question in biomedical research is whether noncoding RNAs are targets of medicines. Bleomycin is a natural product that cleaves DNA; however, it is known to cleave RNA in vitro. Herein, an in-depth analysis of the RNA cleavage preferences of bleomycin?A5 is presented. Bleomycin A5 prefers to cleave RNAs with stretches of AU base pairs. Based on these preferences and bioinformatic analysis, the microRNA-10b hairpin precursor was identified as a potential substrate for bleomycin?A5. Both in vitro and cellular experiments demonstrated cleavage. Importantly, chemical cleavage by bleomycin?A5 in the microRNA-10b hairpin precursors occurred near the Drosha and Dicer enzymatic processing sites and led to destruction of the microRNA. Evidently, oncogenic noncoding RNAs can be considered targets of cancer medicines and might elicit their pharmacological effects by targeting noncoding RNA.

SUBMITTER: Angelbello AJ

PROVIDER: S-EPMC5810124 | biostudies-other | 2018 Jan

REPOSITORIES: biostudies-other

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Bleomycin Can Cleave an Oncogenic Noncoding RNA.

Angelbello Alicia J AJ Disney Matthew D MD

Chembiochem : a European journal of chemical biology 20171122 1

Noncoding RNAs are pervasive in cells and contribute to diseases such as cancer. A question in biomedical research is whether noncoding RNAs are targets of medicines. Bleomycin is a natural product that cleaves DNA; however, it is known to cleave RNA in vitro. Herein, an in-depth analysis of the RNA cleavage preferences of bleomycin A5 is presented. Bleomycin A5 prefers to cleave RNAs with stretches of AU base pairs. Based on these preferences and bioinformatic analysis, the microRNA-10b hairpin ...[more]

PMID: 29084369

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