Ontology highlight
ABSTRACT:
SUBMITTER: Sun H
PROVIDER: S-EPMC5887810 | biostudies-other | 2018 Feb
REPOSITORIES: biostudies-other
Sun Hao H Meledin Roman R Mali Sachitanand M SM Brik Ashraf A
Chemical science 20180111 6
Ester-linked ubiquitinated proteins have been reported by several groups to be involved in ubiquitin signalling. However, due to the lack of the suitable tools to homogeneously produce such conjugates, their exact physiological roles and biochemical behavior remain enigmatic. Here, we report for the first time on the development of a novel synthetic strategy based on total chemical synthesis of proteins to construct ubiquitinated proteins, where ubiquitin is linked to the substrate <i>via</i> an ...[more]