Ontology highlight
ABSTRACT:
SUBMITTER: Dai DP
PROVIDER: S-EPMC5910836 | biostudies-other | 2018 Apr
REPOSITORIES: biostudies-other
Proceedings of the National Academy of Sciences of the United States of America 20180402 16
Reactive oxygen species formed within the mammalian cell can produce 8-oxo-7,8-dihydroguanine (8-oxoG) in mRNA, which can cause base mispairing during gene expression. Here we found that administration of 8-oxoGTP in MTH1-knockdown cells results in increased 8-oxoG content in mRNA. Under this condition, an amber mutation of the reporter luciferase is suppressed. Using second-generation sequencing techniques, we found that U-to-G changes at preassigned sites of the luciferase transcript increased ...[more]