Ontology highlight
ABSTRACT:
SUBMITTER: Hagberg CE
PROVIDER: S-EPMC6137819 | biostudies-other | 2018 Sep
REPOSITORIES: biostudies-other
Hagberg Carolina E CE Li Qian Q Kutschke Maria M Bhowmick Debajit D Kiss Endre E Shabalina Irina G IG Harms Matthew J MJ Shilkova Olga O Kozina Viviana V Nedergaard Jan J Boucher Jeremie J Thorell Anders A Spalding Kirsty L KL
Cell reports 20180901 10
Adipocytes, once considered simple lipid-storing cells, are rapidly emerging as complex cells with many biologically diverse functions. A powerful high-throughput method for analyzing single cells is flow cytometry. Several groups have attempted to analyze and sort freshly isolated adipocytes; however, using an adipocyte-specific reporter mouse, we demonstrate that these studies fail to detect the majority of white adipocytes. We define critical settings required for adipocyte flow cytometry and ...[more]