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Exploration and application of a highly sensitive bis(salamo)-based fluorescent sensor for B4O72- in water-containing systems and living cells.

ABSTRACT: A highly selective fluorescent sensor H4L based on a bis(salamo)-type compound with two N2O2 chelating moieties as ionophore was successfully developed. Sensor H4L was found to have excellent selectivity for B4O72- over many other anions (Br-, CI-, CN-, CO32-, HCO3-, H2PO4-, HSO4-, NO3-, OAc-, S2O3-, SCN-, SO42-, Hcy (homocysteine) and H2O2), and it exhibited an approximately 150-fold enhancement of the fluorescence response to B4O72- in Tris-HCl buffer (DMF/H2O?=?9:1, v/v, pH?=?7) solutions. Significantly, its fluorescence intensity was enhanced in a linear fashion with increasing concentrations of B4O72-. The detection limit of sensor H4L towards B4O72- was 8.61?×?10-7 M. The test strips could conveniently, efficiently and simply detect B4O72- ions in Tris-HCl buffer (DMF/H2O?=?9:1, v/v, pH?=?7) solutions. Furthermore, sensor H4L showed excellent membrane permeability in living cells, and it was successfully used to monitor intracellular B4O72- by confocal luminescence imaging.

SUBMITTER: Pu LM

PROVIDER: S-EPMC6145891 | biostudies-other | 2018 Sep

REPOSITORIES: biostudies-other

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Exploration and application of a highly sensitive bis(salamo)-based fluorescent sensor for B4O72- in water-containing systems and living cells.

Pu Lu-Mei LM Li Xiao-Yan XY Hao Jing J Sun Yin-Xia YX Zhang Yang Y Long Hai-Tao HT Dong Wen-Kui WK

Scientific reports 20180919 1

A highly selective fluorescent sensor H4L based on a bis(salamo)-type compound with two N2O2 chelating moieties as ionophore was successfully developed. Sensor H4L was found to have excellent selectivity for B4O72- over many other anions (Br-, CI-, CN-, CO32-, HCO3-, H2PO4-, HSO4-, NO3 ...[more]

PMID: 30232405

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Project description:As a notorious toxin, formaldehyde (FA) poses an immense threat to human health. Aberrantly elevated FA levels lead to serious pathologies, including organ damage, neurodegeneration, and cancer. Unfortunately, current techniques limit FA imaging to general comparative studies, instead of a mechanistic exploration of its biological role, and this is presumably due to the lack of robust molecular tools for reporting FA in living systems. More importantly, despite being reductive, FA, however, can induce oxidative damage to organisms, thus providing a challenge to the mechanistic study of FA using fluorescence imaging. Herein, we presented the design and multi-application of a bright sensitive ratiometric fluorescent probe 1-(4-(1H-phenanthro[9,10-d]imidazol-2-yl)phenyl) but-3-en-1-amine (PIPBA). With a ?-extended phenylphenanthroimidazole fluorophore and an allylamine group, PIPBA exhibited high quantum yield (? = 0.62) in blue fluorescent emission and selective reactivity toward FA. When sensing FA, PIPBA transformed to PIBE, which is a product capable of releasing bright green fluorescence (? = 0.51) with its enhanced intramolecular charge transfer (ICT). Transformation of PIPBA to PIBE contributed to 80 nm of red shift in emission wavelength and a highly sensitive ratiometric response (92.2-fold), as well as a quite low detection limit (0.84 ?M). PIPBA was successfully applied to various living systems, realizing, for the first time, ratiometric quantification (in cells), in vivo imaging (zebrafish), and living tissue imaging (vivisectional mouse under anaesthetic) of endogenous FA that was spontaneously generated by biological systems. Furthermore, with the aid of PIPBA, we obtained visual evidence for the oxidative damage of FA in both HeLa cells and renal tissue of a living mouse. The results demonstrated that FA exerted indirect oxidative damage by interacting with free radicals, thus producing more oxidizing species, which eventually caused aggravated oxidative damage to the organism. The indirect oxidative damage due to FA could be alleviated by an exogenous or endogenous antioxidant. The excellent behaviors of PIPBA demonstrate that a chemical probe can detect endogenous FA in cells/tissue/vivo, promising to be an effective tool for further exploration of the biological mechanism of FA in living systems.

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Exploration and application of a highly sensitive bis(salamo)-based fluorescent sensor for B4O72- in water-containing systems and living cells.

Publications

Exploration and application of a highly sensitive bis(salamo)-based fluorescent sensor for B<sub>4</sub>O<sub>7</sub><sup>2-</sup> in water-containing systems and living cells.

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