Browse
Submit Data
Databases
API
Help

Dataset Information

27 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Highly sensitive "turn-on" fluorescent sensor for Hg2+ in aqueous solution based on structure-switching DNA.

ABSTRACT: A simple design of "turn-on" fluorescent sensor for mercury was demonstrated based on structure-switching DNA with a low detection limit of 3.2 nM and high selectivity.

SUBMITTER: Wang Z

PROVIDER: S-EPMC3405163 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Highly sensitive "turn-on" fluorescent sensor for Hg2+ in aqueous solution based on structure-switching DNA.

Wang Zidong Z Heon Lee Jung J Lu Yi Y

Chemical communications (Cambridge, England) 20081014 45

A simple design of "turn-on" fluorescent sensor for mercury was demonstrated based on structure-switching DNA with a low detection limit of 3.2 nM and high selectivity. ...[more]

PMID: 19030567

Similar Datasets

Anthracene-Based Highly Selective and Sensitive Fluorescent "Turn-on" Chemodosimeter for Hg2.

Project description:Three ?-extended anthracene-bearing thioacetals (1-3) have been synthesized, and their fluorescence "turn-on" responses to Hg2+ ions are studied. The chemodosimetric fluorescence-sensing behavior and their resulting hydrolysis via a desulfurization reaction mechanism leads to the formation of highly fluorescent respective aldehyde substitutions. Furthermore, this mechanism was supported by increase in the quantum yields of their resulting aldehydes and is correlated to their molecular substitution. The chemosensors 1-3 have exhibited to be promising receptors toward Hg2+ ions in the presence of other competitive metal ions. Moreover, the detection limits of 1-3 have been found to be in the nanomolar range (94, 59, and 235, respectively). Fluorescence microscopic imaging studies show that 1-2 have been found to be effective for fluorescence imaging in live cells. Moreover, compounds 1-3 act as potential candidates for the detection of Hg2+ in environmental and biological systems as well as real samples.

| S-EPMC6217515 | biostudies-literature

A Highly Selective and Sensitive Fluorescent Chemosensor for Detecting Al3+ Ion in Aqueous Solution and Plant Systems.

Project description:The solubilized form of aluminum, Al3+, is present under acid soil conditions and toxic to both animals and plants. Detecting and quantifying Al3+ is vital for both chemistry and biology. A new Schiff-based fluorescent turn-on sensor (probe L) for the selective detection of the Al3+ ion was synthesized by coupling 2-hydroxy-1-naphthaldehyde and 2-aminoisoindoline-1,3-dione, and the structure was characterized by nuclear magnetic resonance spectra. The probe L exhibited an excellent selective and sensitive response to the Al3+ ion over other metal ions in DMSO-H?O (1:9 v/v). Fluorescence quantification revealed that probe L was promising for the detection and accumulation of Al3+. Treating rice seedlings with Al3+ at 25?200 ?M inhibited their growth. Al3+ treatment produced reactive oxygen species in rice roots. Practical applications of the fluorescent probe for the quantification of Al3+ in water samples and rice seedlings are demonstrated. Detecting the Al3+ ion with the probe L is easy and a potential alternative to existing analytical methods. The method can be used for detecting the Al3+ content of aqueous solution and plant systems. The novel fluorescent probe L has good potential for monitoring Al3+ content in the environment and biological systems.

| S-EPMC6387123 | biostudies-literature

Multimodal Fluorescent Polymer Sensor for Highly Sensitive Detection of Nitroaromatics.

Project description:Detection of nitroaromatic explosives with high sensitivity and selectivity is extremely important for civilian and military safety. Here, we report the synthesis and multimodal sensing applications of an emissive alanine based dansyl tagged copolymer P(MMA-co-Dansyl-Ala-HEMA) (DCP), synthesized by RAFT copolymerization. The fluorescent co-polymer exhibited high sensitivity and selectivity towards conventional nitroaromatic explosives such as DNT, TNT and TNP in solution at lower range of µM level and also with saturated vapor of NACs. The quantum yield of the co-polymer was measured to be very high (?f?=?77%) which make it an ideal candidate for sensing in solution as well as in vapor phase. The fluorescence signal from DCP copolymer gets significantly quenched upon addition of aliquots of DNT, TNT, and TNP. The Stern-Volmer constant was calculated to be very high. The quenching mechanism was further established by fluorescence up-conversion, time-resolved fluorescence and steady state absorption spectroscopy. The energetics of sensing process was calculated by Density Functional Theory (DFT) studies. We also fabricate a thin film polymer sensor which was able to detect nitroaromatic vapors with high selectivity. This opens up the possibility of building a low-cost and light-weight nitroaromatic explosives sensor for field use.

| S-EPMC6514181 | biostudies-literature

Novel "Turn-On" Fluorescent Probe for Highly Selectively Sensing Fluoride in Aqueous Solution Based on Tb<sup>3+</sup>-Functionalized Metal-Organic Frameworks.

Project description:A Zr-based metal-organic framework (Zr-MOF) which has free carbonyl groups is synthesized successfully through mix-ligand strategy. Subsequently, Tb3+ is encapsulated into a Zr-MOF by postcoordinated modification. The Tb3+@Zr-MOF exhibits the characteristic emission of Tb3+ because of efficient sensitization through antenna effects. The Tb3+@Zr-MOF is further developed as a novel "turn-on" fluorescent probe to detect fluoride ions in aqueous solution. The results show that Tb3+@Zr-MOF exhibits excellent selectivity, high stability, low detection limits, and good anti-interference for sensitizing fluoride ions. In addition, the possible sensing mechanism that the induced luminescence properties may be attributed to Lewis acid-base interactions is discussed.

| S-EPMC6645131 | biostudies-literature

A Highly Selective Turn-on and Reversible Fluorescent Chemosensor for Al3+ Detection Based on Novel Salicylidene Schiff Base-Terminated PEG in Pure Aqueous Solution.

Project description:The development of highly selective and sensitive chemosensors for Al3+ detection in pure aqueous solution is still a significant challenge. In this work, a novel water-soluble polymer PEGBAB based on salicylidene Schiff base has been designed and synthesized as a turn-on fluorescent chemosensor for the detection of Al3+ in 100% aqueous solution. PEGBAB exhibited high sensitivity and selectivity to Al3+ over other competitive metal ions with the detection limit as low as 4.05 × 10-9 M. PEGBAB displayed high selectivity to Al3+ in the pH range of 5⁻10. The fluorescence response of PEGBAB to Al3+ was reversible in the presence of ethylenediaminetetraacetic acid (EDTA). Based on the fluorescence response, an INHIBIT logic gate was constructed with Al3+ and EDTA as two inputs. Moreover, test strips based on PEGBAB were fabricated facilely for convenient on-site detection of Al3+.

| S-EPMC6523932 | biostudies-literature

Highly compressed water structure observed in a perchlorate aqueous solution.

Project description:The discovery by the Phoenix Lander of calcium and magnesium perchlorates in Martian soil samples has fueled much speculation that flows of perchlorate brines might be the cause of the observed channeling and weathering in the surface. Here, we study the structure of a mimetic of Martian water, magnesium perchlorate aqueous solution at its eutectic composition, using neutron diffraction in combination with hydrogen isotope labeling and empirical potential structure refinement. We find that the tetrahedral structure of water is heavily perturbed, the effect being equivalent to pressurizing pure water to pressures of order 2?GPa or more. The Mg2+ and ClO4- ions appear charge-ordered, confining the water on length scales of order 9?Å, preventing ice formation at low temperature. This may explain the low evaporation rates and high deliquescence of these salt solutions, which are essential for stability within the low relative humidity environment of the Martian atmosphere.Significant amounts of different perchlorate salts have been discovered on the surface of Mars. Here, the authors show that magnesium perchlorate has a major impact on water structure in solution, providing insight into how an aqueous fluid might exist under the sub-freezing conditions present on Mars.

| S-EPMC5715023 | biostudies-literature

A highly selective turn-on colorimetric, red fluorescent sensor for detecting mobile zinc in living cells.

Project description:We describe ZRL1, a turn-on colorimetric and red fluorescent zinc ion sensor. The Zn(2+)-promoted ring opening of the rhodamine spirolactam ring in ZRL1 evokes a 220-fold fluorescence turn-on response. In aqueous media, ZRL1 turn-on luminescence is highly selective for Zn(2+) ions, with no significant response to other competitive cations, including Na(+), K(+), Ca(2+), Mg(2+), Mn(2+), Fe(2+), Co(2+), Ni(2+), Cu(2+), Cd(2+), or Hg(2+). In addition to these characteristics, preliminary results indicate that ZRL1 can be delivered to living cells and can be used to monitor changes in intracellular Zn(2+) levels.

| S-EPMC2993786 | biostudies-literature

A Near-infrared Turn-on Fluorescent Sensor for Sensitive and Specific Detection of Albumin from Urine Samples.

Project description:A readily synthesizable fluorescent probe DMAT-?-CAP was evaluated for sensitive and selective detection of human serum albumin (HSA). DMAT-?-CAP showed selective turn-on fluorescence at 730 nm in the presence of HSA with more than 720-fold enhancement in emission intensity ([DMAT-?-CAP] = 10 ?M), and rapid detection of HSA was accomplished in 3 seconds. The fluorescence intensity of DMAT-?-CAP was shown to increase in HSA concentration-dependent manner (Kd = 15.4 ± 3.3 ?M), and the limit of detection of DMAT-?-CAP was determined to be 10.9 nM (0.72 mg/L). The 1:1 stoichiometry between DMAT-?-CAP and HSA was determined, and the displacement assay revealed that DMAT-?-CAP competes with hemin for the unique binding site, which rarely accommodates drugs and endogenous compounds. Based on the HSA-selective turn-on NIR fluorescence property as well as the unique binding site, DMAT-?-CAP was anticipated to serve as a fluorescence sensor for quantitative detection of the HSA level in biological samples with minimized background interference. Thus, urine samples were directly analyzed by DMAT-?-CAP to assess albumin levels, and the results were comparable to those obtained from immunoassay. The similar sensitivity and specificity to the immunoassay along with the simple, cost-effective, and fast detection of HSA warrants practical application of the NIR fluorescent albumin sensor, DMAT-?-CAP, in the analysis of albumin levels in various biological environments.

| S-EPMC7070906 | biostudies-literature

A Highly Selective and Sensitive Fluorescent Turn-Off Probe for Cu2+ Based on a Guanidine Derivative.

Project description:A new highly selective and sensitive fluorescent probe for Cu2+, N-n-butyl-4-(1'-cyclooctene-1',3',6'-triazole)-1,8-naphthalimide (L), was synthesized and evaluated. The structure of compound L was characterized via IR, ¹H-NMR, 13C-NMR and HRMS. The fluorescent probe was quenched by Cu2+ with a 1:1 binding ratio and behaved as a "turn-off" sensor. An efficient and sensitive spectrofluorometric method was developed for detecting and estimating trace levels of Cu2+ in EtOH/H?O. The ligand exhibited excitation and emission maxima at 447 and 518 nm, respectively. The equilibrium binding constant of the ligand with Cu2+ was 1.57 × 10? M-1, as calculated using the Stern.

| S-EPMC6151758 | biostudies-literature

Highly Sensitive Dansyl-Based Chemosensor for Detection of Cu2+ in Aqueous Solution and Zebrafish.

Project description:A new dansyl-based chemosensor (2-(4-((5-(dimethylamino)naphthalen-1-yl)sulfonyl)piperazin-1-yl)-N-(quinolin-8-yl)acetamide) (DC) for detecting Cu2+ was synthesized and characterized. DC showed great selectivity to Cu2+ by a fluorescent "on-off" detection method. Job plot, ESI-mass spectroscopy, and 1H NMR titration suggested a 1 to 1 binding mode between DC and Cu2+. The detection limit was determined to be 43 nM, which is greatly below the WHO guidelines. In addition, DC can be applied to real samples and zebrafish imaging. The fluorescence quenching mechanism was proposed as the enhancement of intramolecular charge transfer with calculations.

| S-EPMC6682132 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data