Active components with inhibitory activities on IFN-?/STAT1 and IL-6/STAT3 signaling pathways from Caulis Trachelospermi.

ABSTRACT: Initial investigation for new active herbal extract with inhibiting activity on JAK/STAT signaling pathway revealed that the extract of Caulis Trachelospermi, which was separated by 80% alcohol extraction and subsequent HP-20 macroporous resin column chromatography, was founded to strongly inhibit IFN-?-induced STAT1-responsive luciferase activity (IFN-?/STAT1) with IC50 value of 2.43 ?g/mL as well as inhibiting IL-6-induced STAT3-responsive luciferase activity (IL-6/STAT3) with IC50 value of 1.38 ?g/mL. Subsequent study on its active components led to the isolation and identification of two new dibenzylbutyrolactone lignans named 4-demethyltraxillaside (1) and nortrachelogenin 4-O-?-D-glucopyranoside (2), together with six known compounds. The lignan compounds 1-4 together with other lignan compounds isolated in previous study were tested the activities on IFN-?/STAT1 and IL-6/STAT3 pathways. The following result showed that the main components trachelogenin and arctigenin had corresponding activities on IFN-?/STAT1 pathway with IC50 values of 3.14 ?M and 9.46 ?M as well as trachelogenin, arctigenin and matairesinol strongly inhibiting IL-6/STAT3 pathway with IC50 values of 3.63 ?M, 6.47 ?M and 2.92 ?M, respectively.

SUBMITTER: Liu XT 

PROVIDER: S-EPMC6270818 | biostudies-other | 2014 Aug

REPOSITORIES: biostudies-other