Ontology highlight
ABSTRACT:
SUBMITTER: Tanaka J
PROVIDER: S-EPMC6384904 | biostudies-other | 2019 Feb
REPOSITORIES: biostudies-other
Tanaka Junko J Nakagawa Tatsuo T Shiratori Akiko A Shimazaki Yuzuru Y Uematsu Chihiro C Kamahori Masao M Yokoi Takahide T Harada Kunio K Kohara Yoshinobu Y
Scientific reports 20190222 1
Digital PCR (dPCR) has been developed as a method that can quantify nucleic acids more sensitively than real-time PCR. However, dPCR exhibits large fluctuations in the fluorescence intensity of the compartment, resulting in low accuracy. The main cause is most likely due to insufficient PCR. In this study, we proposed a new method that combines dPCR with melting curve analysis and applied that method to KRAS genotyping. Since the melting temperature (Tm) of the PCR product hardly depends on the ...[more]