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Plantaricins markedly enhance the effects of traditional antibiotics against Staphylococcus epidermidis.

ABSTRACT: AIM:Bacteriocins are considered as promising alternatives to antibiotics against infections. In this study, the plantaricins (Pln) A, E, F, J and K were investigated for their antimicrobial activity against Staphylococcus epidermidis. MATERIALS & METHODS:The effects on membrane integrity were studied using liposomes and viable bacteria, respectively. RESULTS:We show that PlnEF and PlnJK caused rapid and significant lysis of S. epidermidis, and induced lysis of liposomes. The PlnEF and PlnJK displayed similar mechanisms by targeting and disrupting the bacterial cell membrane. Interestingly, Pln enhanced the effects of different antibiotics by 30- to 500-fold. CONCLUSION:This study shows that Pln in combination with low concentrations of antibiotics is efficient against S. epidermidis and may be developed as potential treatment of infections.

SUBMITTER: Selegard R

PROVIDER: S-EPMC6393846 | biostudies-other | 2019 Feb

REPOSITORIES: biostudies-other

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Plantaricins markedly enhance the effects of traditional antibiotics against Staphylococcus epidermidis.

Selegård Robert R Musa Amani A Nyström Pontus P Aili Daniel D Bengtsson Torbjörn T Khalaf Hazem H

Future microbiology 20190116

<h4>Aim</h4>Bacteriocins are considered as promising alternatives to antibiotics against infections. In this study, the plantaricins (Pln) A, E, F, J and K were investigated for their antimicrobial activity against Staphylococcus epidermidis.<h4>Materials & methods</h4>The effects on membrane integrity were studied using liposomes and viable bacteria, respectively.<h4>Results</h4>We show that PlnEF and PlnJK caused rapid and significant lysis of S. epidermidis, and induced lysis of liposomes. Th ...[more]

PMID: 30648887

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Project description:Staphylococcus epidermidis is implicated in a multitude of human infections and is one of the major causes of clinical infections in hospitals, especially at surgical sites and on indwelling medical devices, such as orthopedic implants. These infections are especially dangerous because of the S. epidermidis propensity to form biofilms, which increases resistance to antibiotics and the natural immune response. This study investigated pulsed electromagnetic fields (PEMF) as a potential treatment to combat such infections, as PEMF exposure was expected to disrupt the electrostatic forces that adhere staphylococcal cells to surfaces and to one another. To test the effect of PEMF on biofilms, S. epidermidis cultures were exposed to PEMF at various durations either during the growth phase or after a full biofilm had formed. In addition, cells were exposed to PEMF and concomitant antibiotic treatment. Biofilm viability was quantified by both crystal violet and alamarBlue assays and scanning electron microscopy. The results demonstrated that PEMF significantly inhibited biofilm formation and disrupted preformed biofilms in vitro while also showing synergistic biofilm inhibition when combined with antibiotics. These combined results indicate that PEMF should be considered a promising novel technique for treating S. epidermidis biofilm infections and undergo further testing in vivo. IMPORTANCE Antibiotic resistance and biofilm infections are major issues in health care because of the lack of a successful treatment modality and poor patient outcomes. These infections are a particular issue following orthopedic surgery or trauma wherein an infection may form on an orthopedic implant or patient's bone. The presented study demonstrates that pulsed electromagnetic fields may be a promising novel treatment for such infections and can overcome the medical challenges presented by biofilm formation. Furthermore, the effects demonstrated are even greater when combining pulsed electromagnetic field therapy with traditional antibiotics.

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Plantaricins markedly enhance the effects of traditional antibiotics against Staphylococcus epidermidis.

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Plantaricins markedly enhance the effects of traditional antibiotics against Staphylococcus epidermidis.

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