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Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis.

ABSTRACT: INTRODUCTION:Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is a powerful approach for visualizing the localization of metabolites. OBJECTIVES:A method to keep the shape of plant tissue needs to be developed for MALDI-IMS. METHODS:The method was developed using transfer tape and double-sided conductive tape. RESULTS:MALDI-IMS analysis using the developed method enabled to perform segmentation and correlation analysis of mass features. CONCLUSION:This proof-of-concept study showed that rutin localizes in the epidermis, developing tissue, and protoxylem in Asparagus officinalis.

SUBMITTER: Nakabayashi R

PROVIDER: S-EPMC6394462 | biostudies-other | 2019 Feb

REPOSITORIES: biostudies-other

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Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis.

Nakabayashi Ryo R Hashimoto Kei K Toyooka Kiminori K Saito Kazuki K

Metabolomics : Official journal of the Metabolomic Society 20190214 2

<h4>Introduction</h4>Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is a powerful approach for visualizing the localization of metabolites.<h4>Objectives</h4>A method to keep the shape of plant tissue needs to be developed for MALDI-IMS.<h4>Methods</h4>The method was developed using transfer tape and double-sided conductive tape.<h4>Results</h4>MALDI-IMS analysis using the developed method enabled to perform segmentation and correlation analysis of mass feature ...[more]

PMID: 30830429

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