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Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues.

ABSTRACT: Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of clearing protocols, which will facilitate wide adoption by preclinical researchers and clinical laboratories. In particular, the open-top geometry provides unsurpassed versatility to interface with a wide range of accessory technologies in the future.

SUBMITTER: Glaser AK

PROVIDER: S-EPMC6609674 | biostudies-other | 2019 Jul

REPOSITORIES: biostudies-other

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Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues.

Glaser Adam K AK Reder Nicholas P NP Chen Ye Y Yin Chengbo C Wei Linpeng L Kang Soyoung S Barner Lindsey A LA Xie Weisi W McCarty Erin F EF Mao Chenyi C Halpern Aaron R AR Stoltzfus Caleb R CR Daniels Jonathan S JS Gerner Michael Y MY Nicovich Philip R PR Vaughan Joshua C JC True Lawrence D LD Liu Jonathan T C JTC

Nature communications 20190704 1

Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of clearing protocols, which will facilit ...[more]

PMID: 31273194

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