Unknown

Dataset Information

0

Cytomegalovirus-Infected Primary Endothelial Cells Trigger NKG2C+ Natural Killer Cells.


ABSTRACT: Among innate cells, natural killer (NK) cells play a crucial role in the defense against cytomegalovirus (CMV). In some individuals, CMV infection induces the expansion of NKG2C+ NK cells that persist after control of the infection. We have previously shown that KIR2DL+ NK cells, in contrast to NKG2C+ NK cells, contribute to controlling CMV infection using a CMV-infected monocyte-derived dendritic cell (MDDC) model. However, the nature of CMV-infected cells contributing to the expansion of the NKG2C+ NK cell subset remains unclear. To gain more insight into this question, we investigated the contribution of NKG2C+ NK cell activation by CMV-infected primary human aortic endothelial cells (EC) isolated from kidney transplant donors, which constitutively express the human leukocyte antigen (HLA)-E molecule. Here, we show that, although classic HLA class I expression was drastically downregulated, nonclassic HLA-E expression was maintained in CMV-infected EC. By comparing HLA expression patterns in CMV-infected EC, fibroblasts and MDDC, we demonstrate a cell-dependent modulation of HLA-E expression by CMV infection. NKG2C+ NK cell degranulation was significantly triggered by CMV-infected EC regardless of the nature of the HLA-E allele product. EC, predominantly present in vessels, may constitute a privileged site for CMV infection that drives a 'memory' NKG2C+ NK cell subset.

SUBMITTER: Djaoud Z 

PROVIDER: S-EPMC6738823 | biostudies-other | 2016

REPOSITORIES: biostudies-other

altmetric image

Publications

Cytomegalovirus-Infected Primary Endothelial Cells Trigger NKG2C+ Natural Killer Cells.

Djaoud Zakia Z   Riou Raphaëlle R   Gavlovsky Pierre-Jean PJ   Mehlal Souad S   Bressollette Céline C   Gérard Nathalie N   Gagne Katia K   Charreau Béatrice B   Retière Christelle C  

Journal of innate immunity 20160427 4


Among innate cells, natural killer (NK) cells play a crucial role in the defense against cytomegalovirus (CMV). In some individuals, CMV infection induces the expansion of NKG2C+ NK cells that persist after control of the infection. We have previously shown that KIR2DL+ NK cells, in contrast to NKG2C+ NK cells, contribute to controlling CMV infection using a CMV-infected monocyte-derived dendritic cell (MDDC) model. However, the nature of CMV-infected cells contributing to the expansion of the N  ...[more]

Similar Datasets

| S-EPMC2843162 | biostudies-literature
| S-EPMC1988958 | biostudies-literature
| S-EPMC6614060 | biostudies-literature
| S-EPMC2919936 | biostudies-literature
| S-EPMC2842453 | biostudies-literature
| S-EPMC5597263 | biostudies-literature
| S-EPMC3530928 | biostudies-literature
| S-EPMC10014884 | biostudies-literature
| S-EPMC1950948 | biostudies-literature
| S-EPMC4254395 | biostudies-literature