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Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator.

ABSTRACT: A new cyan fluorescent probe, MIPY-DNBS, using an imidazo[1,5-α]pyridine derivative as the fluorophore and 2,4-dinitrobenzensufonate as the recognition site for the selective detection of thiols (Cys, GSH, and Hcy), was designed and synthesized. Probe MIPY-DNBS exhibited a 172 nm Stokes shift, a fast response time (400 s), low cytotoxicity, low detection limits (12.7 nM for Cys), and excellent selectively in the detection of thiols. In addition, MIPY-DNBS was successfully applied to imaging thiols in living MCF-7 cells and zebrafish.

SUBMITTER: Chen S

PROVIDER: S-EPMC6767008 | biostudies-other | 2019 Sep

REPOSITORIES: biostudies-other

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Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator.

Chen Song S Hou Peng P Sun Jingwen J Wang Haijun H Liu Lei L

Molecules (Basel, Switzerland) 20190912 18

A new cyan fluorescent probe, MIPY-DNBS, using an imidazo[1,5-α]pyridine derivative as the fluorophore and 2,4-dinitrobenzensufonate as the recognition site for the selective detection of thiols (Cys, GSH, and Hcy), was designed and synthesized. Probe MIPY-DNBS exhibited a 172 nm Stokes shift, a fast response time (400 s), low cytotoxicity, low detection limits (12.7 nM for Cys), and excellent selectively in the detection of thiols. In addition, MIPY-DNBS was successfully ap ...[more]

PMID: 31547406

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