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PRC2- EZH1 contributes to circadian gene expression by orchestrating chromatin states and RNA polymerase II complex stability

ABSTRACT: Circadian rhythmicity of gene expression is a conserved feature of cell physiology. This involves fine-tuning between transcriptional and post-transcriptional mechanisms and strongly depends on the metabolic state of the cell. Together these processes guarantee an adaptive plasticity of tissue-specific genetic programs. However, it is unclear how the epigenome and RNA Pol II rhythmicity are integrated. Here we show that the PcG protein EZH1 has a gateway bridging function in post-mitotic skeletal muscle cells. On one hand, the circadian clock master regulator BMAL1 directly controls oscillatory behavior and periodic assembly of core components of the PRC2-EZH1 complex. On the other hand, EZH1 is essential for circadian gene expression at alternate Zeitgeber times, through stabilization of RNA Polymerase II preinitiation complexes, thereby controlling nascent transcription. Collectively, our data show that PRC2-EZH1 regulates circadian transcription both negatively and positively by modulating chromatin states and basal transcription complex stability.

SUBMITTER: Prof. Valerio Orlando

PROVIDER: S-SCDT-10_1038-S44318-024-00267-2 | biostudies-other |

REPOSITORIES: biostudies-other

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