In vivo HIV-1 nuclear condensates safeguard against cGAS and license reverse transcription

ABSTRACT: Entry of viral capsids into the nucleus induces the formation of biomolecular condensates called HIV-1 membraneless organelles (HIV-1-MLOs). Several questions remain about their persistence, in vivo formation, composition, and function. Our study reveals that HIV-1-MLOs persisted for several weeks in infected cells, and their abundance correlated with viral infectivity. Using an appropriate animal model, we show that HIV-1-MLOs were formed in vivo during acute infection. To explore the viral structures present within these biomolecular condensates, we used a combination of double immunogold labeling, electron microscopy and tomography, and unveiled a diverse array of viral core structures. Our functional analyses showed that HIV-1-MLOs remained stable during treatment with a reverse transc

SUBMITTER: Francesca Di Nunzio 

PROVIDER: S-SCDT-10_1038-S44318-024-00316-W | biostudies-other |

REPOSITORIES: biostudies-other