Targeted assembly of ectopic kinetochores to induce chromosome-specific segmental aneuploidies

ABSTRACT: Cancer cells display persistent underlying chromosomal instability, with individual tumour types intriguingly exhibiting characteristic subsets of whole, and sub-chromosomal aneuploidies. Few methods to induce specific aneuploidies at will exist, hampering investigation of functional consequences of recurrent aneuploidies, as well as the acute consequences of specific chromosome mis-segregation. We therefore investigated the possibility of sabotaging the mitotic segregation of specific chromosomes using nuclease-dead CRISPR-Cas9 (dCas9) as a cargo carrier to specific genomic loci. We recruited the kinetochore-nucleating domain of centromere protein CENP-T to assemble ectopic kinetochores either near the centromere of chromosome 9, or the telomere of chromosome 1. Ectopic kinetochore assemb

SUBMITTER: Dr. Laura Tovini 

PROVIDER: S-SCDT-10_15252-EMBJ_2022111587 | biostudies-other |

REPOSITORIES: biostudies-other