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RNA Polymerase II CTD is dispensable for transcription and required for termination in human cells

ABSTRACT: The largest subunit of RNA polymerase (Pol) II harbors an evolutionarily conserved C-Terminal-Domain (CTD), composed of heptapeptide repeats, central to the transcriptional process. Here, we analyze the transcriptional phenotypes of a CTD-delta5 mutant that carries a large CTD truncation in human cells. Our data show that this mutant can transcribe genes in living cells but displays a pervasive phenotype with impaired termination, similar to but more severe than previously characterized mutations of CTD tyrosine residues. The CTD-delta5 mutant does not interact with the Mediator and Integrator complexes involved in activation of transcription and processing of RNAs. Examination of long-distance interactions and CTCF binding patterns in CTD-delta5 mutant cells reveals no changes in TAD domains or borders. Our data demonstrate that the CTD is largely dispensable for the act of transcription in living cells. We propose a model in which CTD-depleted Pol II has a lower entry rate onto DNA but becomes pervasive once engaged in transcription, resulting in a defect in termination.

SUBMITTER: Ms. Yousra Yahia

PROVIDER: S-SCDT-10_15252-EMBR_202256150 | biostudies-other |

REPOSITORIES: biostudies-other

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