Let-7a-regulated translational readthrough of mammalian AGO1 generates a microRNA pathway inhibitor

ABSTRACT: Translational readthrough generates proteins with extended C terminus which often possess distinct properties. Here, we have demonstrated translational readthrough of AGO1 using various reporter assays. Analysis of ribosome profiling data and mass-spectrometry data provided additional evidences for translational readthrough of AGO1. We could also detect endogenous readthrough product, Ago1x, using a specific antibody both in vitro and in vivo. This process is programmed by a cis sequence downstream to the canonical stop codon of AGO1 which could drive readthrough even in a heterologous context. The cis sequence has a let-7a miRNA binding site and the readthrough process is promoted by let-7a miRNA. Interestingly, Ago1x can load miRNAs on target mRNAs without causing post-transcriptional ge

SUBMITTER: Anumeha Singh 

PROVIDER: S-SCDT-EMBOJ-2018-100727 | biostudies-other |

REPOSITORIES: biostudies-other