POLO REGULATES SPINDLY TO PREVENT PREMATURE STABILIZATION OF KINETOCHORE-MICROTUBULE ATTACHMENTS

ABSTRACT: Accurate chromosome segregation in mitosis requires sister kinetochores to bind to microtubules from opposite spindle poles. The stability of kinetochore-microtubule attachments is fine-tuned to prevent or correct erroneous attachments while preserving amphitelic interactions. Polo kinase has been implicated both in stabilizing and destabilizing kinetochore-microtubule attachments. However, the mechanism underlying Polo-destabilizing activity remains elusive. Here, resorting to an RNAi-screen in Drosophila for suppressors of a constitutively active Polo mutant, we identified a strong genetic interaction between Polo and the Rod-ZW10-Zwilch (RZZ) complex, whose kinetochore accumulation has been shown to antagonize microtubule stability. We find that Polo phosphorylates Spindly and impairs i

SUBMITTER: João Barbosa 

PROVIDER: S-SCDT-EMBOJ-2018-100789 | biostudies-other |

REPOSITORIES: biostudies-other