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MiT/TFE factors control ER-phagy via transcriptional regulation of FAM134B


ABSTRACT: Lysosomal degradation of the endoplasmic reticulum (ER) via autophagy (ER-phagy) is emerging as a critical regulator of cell homeostasis and function. The recent identification of ER-phagy receptors has shed light on the molecular mechanisms underlining this process. However, the signalling pathways regulating ER-phagy in response to cellular needs are still largely unknown. We found that the nutrient responsive transcription factors TFEB and TFE3 - master regulators of lysosomal biogenesis and autophagy- control ER-phagy by inducing the expression of the ER-phagy receptor FAM134B. The TFEB/TFE3-FAM134B axis promotes ER-phagy activation upon prolonged starvation. In addition, that this pathway is activated in chondrocytes by FGF signalling, a critical regulator of skeletal growth. FGF signalling induces JNK-dependent proteasomal degradation of the insulin receptor substrate 1 (IRS1), which in turn inhibits the PI3K-PKB/Akt-mTORC1 pathway and promotes TFEB/TFE3 nuclear translocation and enhances FAM134B transcription. Notably, FAM134B is required for protein secretion in chondrocytes, and cartilage growth and bone mineralization in medaka fish. This study identifies a new signalling pathway that allows ER-phagy to respond to both metabolic and developmental cues.

SUBMITTER: Laura Cinque 

PROVIDER: S-SCDT-EMBOJ-2020-105696P | biostudies-other |

REPOSITORIES: biostudies-other

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